LOCUS       pDONR™/Zeo
DISCLAIMER  Certain terms are trademarks or registered trademarks of Invitrogen 
            Corporation. See "Intellectual Property" in the Help file for more 
            information.
FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(1643..1739)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(1754..1773)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     1781..1797
                     /note="M13 Reverse priming site"
     gene            1910..2719
                     /note="Kanamycin resistance gene"
     rep_origin      2840..3513
                     /note="pUC origin"
     vector          join(1651..3516,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    669..691
                     /note="TEV site"
     source          692..1636
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:13319 IMAGE:4042813"
                     /tissue_type="Lung"
                     /clone_lib="NIH_MGC_77"
                     /lab_host="DH10B"
                     /note="Vector: pDNR-LIB"
     gene            692..1636
                     /gene="CHST11"
                     /gene_synonym=C4ST
                     /gene_synonym=C4ST-1
                     /gene_synonym=C4ST1
                     /gene_synonym=HSA269537
                     /db_xref="GeneID:50515"
                     /db_xref="HGNC:17422"
                     /db_xref="MIM:610128"
     CDS             692..1636
                     /dnas_title="carbohydrate (chondroitin 4)
                     sulfotransferase 11"
                     /gene="CHST11"
                     /gene_synonym=C4ST
                     /gene_synonym=C4ST-1
                     /gene_synonym=C4ST1
                     /gene_synonym=HSA269537
                     /codon_start=1
                     /product="carbohydrate (chondroitin 4) sulfotransferase
                     11"
                     /protein_id="AAH13315.1"
                     /db_xref="GI:15426430"
                     /db_xref="GeneID:50515"
                     /db_xref="HGNC:17422"
                     /db_xref="MIM:610128"
                     /translation="MKPALLEVMRMNRICRMVLATCLGSFILVIFYFQSMLHPVMRRN
                     PFGVDICCRKGSRSPLQELYNPIQLELSNTAVLHQMRRDQVTDTCRANSATSRKRRVL
                     TPNDLKHLVVDEDHELIYCYVPKVACTNWKRLMMVLTGRGKYSDPMEIPANEAHVSAN
                     LKTLNQYSIPEINHRLKSYMKFLFVREPFERLVSAYRNKFTQKYNISFHKRYGTKIIK
                     RQRKNATQEALRKGDDVKFEEFVAYLIDPHTQREEPFNEHWQTVYSLCHPCHIHYDLV
                     GKYETLEEDSNYVLQLAGVGSYLKFPTYAKSTRTTDEMTTEFFQNISSEHQTQLYEVY
                     KLDFLMFNYSVPSYLKLE"
     misc_feature    692..1636
                     /note="CHST11 coding region"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg ccacccagtc atgcggagga atccctttgg
        721 tgtggacatc tgctgccgga aggggtcccg aagccccctg caggaactct acaacccaat
        781 ccagctggag ctctcaaaca ctgctgtcct gcaccagatg cggcgggacc aggtgacaga
        841 cacgtgccga gccaacagcg ccacaagccg taagcggagg gtgctgaccc ccaacgacct
        901 gaagcacttg gtggtggatg aggaccacga gctcatctac tgctacgtgc ccaaggtggc
        961 ctgcaccaac tggaagcggc tcatgatggt cctgaccggg cgggggaagt acagcgaccc
       1021 catggagatc ccggccaacg aggcacacgt ctccgccaac ctgaagaccc tgaaccagta
       1081 cagcatccca gaaatcaacc accgcttgaa aagctacatg aagttcctgt ttgtccggga
       1141 gcccttcgag aggctagtgt ccgcctaccg caacaagttc acccagaagt acaacatctc
       1201 cttccacaag cggtacggca ccaagatcat caaacgccag cggaagaacg ccacccagga
       1261 ggccctgcgc aaaggggacg atgtcaaatt cgaggagttt gtggcctatc tcatcgaccc
       1321 acacacccag cgggaggagc ctttcaacga acactggcaa accgtctact cactctgcca
       1381 tccctgccac atccactatg acctcgtggg caagtacgag acactggaag aggattctaa
       1441 ttacgtcctg cagctggcag gagtgggcag ctacctgaag ttccccacct atgcaaagtc
       1501 tacgagaact actgatgaaa tgaccacaga attcttccag aacatcagct cagagcacca
       1561 aacgcagctg tacgaagtct acaaactcga ttttttaatg ttcaattact cagtgccaag
       1621 ctacctgaaa ttggaaTAGG ACCCAGCTTT CTTGTACAAA GTTGGCATTA TAAGAAAGCA
       1681 TTGCTTATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTTG
       1741 CCATCCAGCT GATATCCCCT ATAGTGAGTC GTATTACATG GTCATAGCTG TTTCCTGGCA
       1801 GCTCTGGCCC GTGTCTCAAA ATCTCTGATG TTACATTGCA CAAGATAAAA TAATATCATC
       1861 ATGAACAATA AAACTGTCTG CTTACATAAA CAGTAATACA AGGGGTGTTA TGAGCCATAT
       1921 TCAACGGGAA ACGTCGAGGC CGCGATTAAA TTCCAACATG GATGCTGATT TATATGGGTA
       1981 TAAATGGGCT CGCGATAATG TCGGGCAATC AGGTGCGACA ATCTATCGCT TGTATGGGAA
       2041 GCCCGATGCG CCAGAGTTGT TTCTGAAACA TGGCAAAGGT AGCGTTGCCA ATGATGTTAC
       2101 AGATGAGATG GTCAGACTAA ACTGGCTGAC GGAATTTATG CCTCTTCCGA CCATCAAGCA
       2161 TTTTATCCGT ACTCCTGATG ATGCATGGTT ACTCACCACT GCGATCCCCG GAAAAACAGC
       2221 ATTCCAGGTA TTAGAAGAAT ATCCTGATTC AGGTGAAAAT ATTGTTGATG CGCTGGCAGT
       2281 GTTCCTGCGC CGGTTGCATT CGATTCCTGT TTGTAATTGT CCTTTTAACA GCGATCGCGT
       2341 ATTTCGTCTC GCTCAGGCGC AATCACGAAT GAATAACGGT TTGGTTGATG CGAGTGATTT
       2401 TGATGACGAG CGTAATGGCT GGCCTGTTGA ACAAGTCTGG AAAGAAATGC ATAAACTTTT
       2461 GCCATTCTCA CCGGATTCAG TCGTCACTCA TGGTGATTTC TCACTTGATA ACCTTATTTT
       2521 TGACGAGGGG AAATTAATAG GTTGTATTGA TGTTGGACGA GTCGGAATCG CAGACCGATA
       2581 CCAGGATCTT GCCATCCTAT GGAACTGCCT CGGTGAGTTT TCTCCTTCAT TACAGAAACG
       2641 GCTTTTTCAA AAATATGGTA TTGATAATCC TGATATGAAT AAATTGCAGT TTCATTTGAT
       2701 GCTCGATGAG TTTTTCTAAT CAGAATTGGT TAATTGGTTG TAACACTGGC AGAGCATTAC
       2761 GCTGACTTGA CGGGACGGCG CAAGCTCATG ACCAAAATCC CTTAACGTGA GTTACGCGTC
       2821 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT
       2881 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT
       2941 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT
       3001 ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC
       3061 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA
       3121 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG
       3181 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG
       3241 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG
       3301 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA
       3361 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT
       3421 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG
       3481 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTT
//